Hassle-Free Osteoblasts for Your Research

Bone osteoblasts are integral to bone remodeling and are studied to understand bone pathophysiology and regeneration. These cells can be isolated from the femur of different animal models such as mice, rats, and swine. The process of isolation follows the extraction of a femur sample by surgical methods. Thereafter, the femur is placed in a buffer solution and cleaned from any external tissue. Afterward, the femur is dissected into smaller pieces and transferred into a collagenase type II solution for 30 minutes at room temperature in a Petri dish. In the next step, the collagenase type II solution is replaced by the collagenase type IV solution. After overnight incubation, bone pieces are washed with a buffer, and culture media (DMEM) is added. For attachment, scrape the petridish with forceps. After a week, cells shift from the bone pieces to the culture dish. Now the cells are isolated, they can be cultivated further on getting coated dishes.
But if you want to avoid the long and labor-extensive osteoblasts isolation process, select Kosheeka as your cell provider. We provide primary bone osteoblasts and tailor the species and donor according to your needs. Mail us at info@kosheeka.com.